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Secondary Structure of Organophosphorus Hydrolase in Solution and in Langmuir−Blodgett Film Studied by Circular Dichroism Spectroscopy

✍ Scribed by Zheng, Jiayin; Constantine, Celeste A.; Rastogi, Vipin K.; Cheng, Tu-Chen; DeFrank, Joseph J.; Leblanc, Roger M.

Book ID
126161783
Publisher
American Chemical Society
Year
2004
Tongue
English
Weight
175 KB
Volume
108
Category
Article
ISSN
0022-3654

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Estimates of the secondary structure of a protein in solution are made by mathematical analyses of its circular dichroism (CD) spectrum below 240 nm. All current procedures require accurate determination of the concentration of the protein sample. Insoluble proteins, such as prions or amyloid, are e