Secondary structure dependent on metal ions of copper,zinc superoxide dismutase investigated by Fourier transform IR spectroscopy
✍ Scribed by Wei-Yin Sun; Jiang-Lin Fang; Min Cheng; Ping-Yu Xia; Wen-Xia Tang
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1997
- Tongue
- English
- Weight
- 103 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
Copper,zinc superoxide dismutase (Cu 2 Zn 2 -SOD) from bovine erythrocyte and its metal ion free derivatives, E 2 Zn 2 -SOD, Cu 2 E 2 -SOD, and E 2 E 2 -SOD (E: empty) were prepared and their secondary structures were investigated by Fourier transform ir spectroscopy. In 20 mM deuterated phosphate buffer (pD 7.5) solution at room temperature, the native Cu 2 Zn 2 -SOD contains about 34% b-strand, 17% b-turn, and 49% unordered structures, which is similar to the content determined by x-ray crystal structural analysis. The metal ion free derivatives decrease the component of b-strand and increase the unordered structure component in trend. Especially in the cases of zinc-free derivatives, Cu 2 E 2 -SOD and E 2 E 2 -SOD, about 24% b-strand, 20% b-turn, and 57% unordered structures are obtained. The result indicates that the zinc ion plays an important role in determining the secondary structure of copper,zinc superoxide dismutase.