Se-(8-azidoadenosyl) [75Se]selenomethionine as a photoaffinity label for S-adenosylmethionine binding proteins
✍ Scribed by Dennis R. Warner; Jerald L. Hoffman
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 674 KB
- Volume
- 195
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A method is described for the synthesis and purification of the photoaffinity label Se-(8-azidoadenosyl)[75Se]selenomethionine. This photoaffinity label can be used to specifically and covalently label the S-adenosylmethionine binding site of proteins that use this cofactor, as exemplified by labeling of thioether methyltransferase. By utilizing the gamma-emitting isotope of selenium, Se-(8-azidoadenosyl)[75Se]selenomethionine eliminates the need for the impregnation of acrylamide gels with fluorographic enhancers and dilution of liquid samples into scintillation cocktails, as is required with the commonly used methyl-3H-labeled and 35S-labeled S-(8-azidoadenosyl)methionine.