SDS disc electrophoresis of proteins in homogeneous, low-concentrated polyacrylamide gels
β Scribed by I. Piotr Maly; Cordula Nitsch
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 429 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0173-0835
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A method is described which permits the purification of proteins in quantities necessary for physicochemical characterization. The protein to be purified is separated from contaminants by electrophoresis in a number of sodium dodecyl sulfate (SDS)-containing polyacrylamide disc gels. The region of e
A simple method for the quantitation of proteins on SDS-polyacrylamide gels, with lysozyme as an internal standard, has been designed. Gels containing known weight ratios of standard proteins to lysozyme were electrophoresed, stained with Coomassie blue R250, and scanned at 550 nm. Peak areas corres