𝔖 Bobbio Scriptorium
✦   LIBER   ✦

SDS disc electrophoresis of proteins in homogeneous, low-concentrated polyacrylamide gels

✍ Scribed by I. Piotr Maly; Cordula Nitsch


Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
429 KB
Volume
28
Category
Article
ISSN
0173-0835

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Recovery of SDS-proteins from polyacryla
✍ Barry R. Ziola; Douglas G. Scraba πŸ“‚ Article πŸ“… 1976 πŸ› Elsevier Science 🌐 English βš– 394 KB

A method is described which permits the purification of proteins in quantities necessary for physicochemical characterization. The protein to be purified is separated from contaminants by electrophoresis in a number of sodium dodecyl sulfate (SDS)-containing polyacrylamide disc gels. The region of e

Quantitation of stained proteins in SDS
✍ Wen-Ching Hsieh; Robert E. Anderson πŸ“‚ Article πŸ“… 1975 πŸ› Elsevier Science 🌐 English βš– 437 KB

A simple method for the quantitation of proteins on SDS-polyacrylamide gels, with lysozyme as an internal standard, has been designed. Gels containing known weight ratios of standard proteins to lysozyme were electrophoresed, stained with Coomassie blue R250, and scanned at 550 nm. Peak areas corres