SCX separation of recombinant thrombolytic protein from complex biological feeds

✍ Scribed by Young J. Kim

• Book ID: 104649323
• Publisher: Springer-Verlag
• Year: 1995
• Tongue: English
• Weight: 456 KB
• Volume: 9
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00160829

✦ Synopsis

The purification of recombinant thrombolytic protein from a crude fermentation broth mixture was examined in strong cation exchange (SCX) chromatographic system. SCX system was shown to exhibit unique selectivity for the recombinant protein broth mixture.

While SCX system showed lower total binding capacity for the recombinant protein than Anti-body Exchange system, it showed sharper separation boundaries in both displacement and step gradient module. The research with the SCX indicated that this separating system could be successfully employed in the displacement mode using DEAE-dextran as the displacer.