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Screening populations of individual cells for secretory heterogeneity

✍ Scribed by Craig A. Aspinwall; Edward S. Yeung

Book ID
105889207
Publisher
Springer
Year
2004
Tongue
English
Weight
376 KB
Volume
381
Category
Article
ISSN
1618-2650

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We have developed a rapid and sensitive screening method to detect the secretory product from transformed secretory cells. Polyclonal antibodies against the target secretory protein are first adsorbed onto a nitrocellulose membrane and are then called solid-phase antibodies. Then this membrane is pl