We have screened the p53 status of 156 human cell lines, including 142 tumor cell lines from 27 different tumor types and 14 cell lines from normal tissues by using functional analysis of separated alleles in yeast. This assay enables us to score wild-type p53 expression on the basis of the ability
Screening patients for heterozygous p53 mutations using a functional assay in yeast
β Scribed by Ishioka, Chikashi; Frebourg, Thierry; Yan, Yu-Xin; Vidal, Marc; Friend, Stephen H.; Schmidt, Susanne; Iggo, Richard
- Book ID
- 109918215
- Publisher
- Nature Publishing Group
- Year
- 1993
- Tongue
- English
- Weight
- 691 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1061-4036
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Although p53-gene mutations occur with significant frequency in diffuse low-grade and high-grade astrocytomas, and are postulated to play an important role in tumorigenesis in these cases, the role of the p53 gene in pilocytic astrocytomas remains unclear. Published data using DNA-based assays for p
p53 status was tested in 180 patients with primary breast cancer using a yeast functional assay. Mutations were identified in 32% of cases. Only half were point missense mutations; the remainder were nonsense, insertion, deletion and splice site mutations. Twenty-two percent of mutations were locate