Screening of T7 phage displayed Bactrocera dorsalis (Hendel) antenna cDNA library against chemosensory protein
✍ Scribed by Liming Hu; Jianmei Shen; Meiying Hu; Muhammad Rizwan-ul-Haq; Weining Hao
- Book ID
- 102745146
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 552 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0739-4462
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✦ Synopsis
Abstract
Recent studies have shown that chemosensory proteins (CSPs) were involved in diverse life activities such as insect feeding, development, mating, immune regulation, as well as other important circadian rhythms, etc. To screen the proteins involved in the __Bdor__CSP‐related physiological activity, a cDNA library of the Bactrocera dorsalis (Hendel) antenna expressed on the surface of T7 phage was screened against __Bdor__CSP. After four rounds of screening, ELISA‐positive samples of selected phages were sequenced and identified as protein disulfide isomerase (PDI), trypsin‐like serine protease (Ser), TakeOut (TO), and a new protein by GenBank blast, respectively. Real‐time quantitative PCR results showed that the expression levels of Ser, TO, and the new protein were the highest in antenna, sharing similar expression pattern with __Bdor__CSP. These results reveal that these proteins might be involved in the __Bdor__CSP‐related physiological or metabolic activities. This work paves a new way for exploring the function of CSPs. © 2010 Wiley Periodicals, Inc.