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Screening of supports for the immobilization of pectinmethylesterase from acerola (Malpighia glabra L)

✍ Scribed by Sandra Aparecida de Assis; Pedro Fernandes; Bruno Sommer Ferreira; Joaquim MS Cabral; Olga Maria Mascarenhas Faria Oliveira


Publisher
Wiley (John Wiley & Sons)
Year
2004
Tongue
English
Weight
86 KB
Volume
79
Category
Article
ISSN
0268-2575

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✦ Synopsis


Abstract

A partially purified extract of pectinmethylesterase (PME) from acerola fruit was immobilized on various supports: glass, celite, chrysotile, agarose, concanavalin A Sepharose 4B, egg shell, polyacrylamide and gelatin. In addition, reticulation with glutaraldehyde was assessed, as well as the use of gelatin in the presence of celite, glass and silica. The highest immobilization yields were obtained when the pectinmethylesterase was immobilized in concanavalin A Sepharose 4B (81.7%) and in gelatin‐water (78.0%). Copyright Β© 2004 Society of Chemical Industry


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