## Abstract The aim of this work was to develop an efficient reactor for the production of low methoxyl pectin, using pectinmethylesterase (PME, EC 3.1.1.11) from acerola immobilized on silica. The immobilized enzyme was used in up to 50 successive bioconversion runs at 50 Β°C with an efficiency los
Screening of supports for the immobilization of pectinmethylesterase from acerola (Malpighia glabra L)
β Scribed by Sandra Aparecida de Assis; Pedro Fernandes; Bruno Sommer Ferreira; Joaquim MS Cabral; Olga Maria Mascarenhas Faria Oliveira
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2004
- Tongue
- English
- Weight
- 86 KB
- Volume
- 79
- Category
- Article
- ISSN
- 0268-2575
- DOI
- 10.1002/jctb.973
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β¦ Synopsis
Abstract
A partially purified extract of pectinmethylesterase (PME) from acerola fruit was immobilized on various supports: glass, celite, chrysotile, agarose, concanavalin A Sepharose 4B, egg shell, polyacrylamide and gelatin. In addition, reticulation with glutaraldehyde was assessed, as well as the use of gelatin in the presence of celite, glass and silica. The highest immobilization yields were obtained when the pectinmethylesterase was immobilized in concanavalin A Sepharose 4B (81.7%) and in gelatinβwater (78.0%). Copyright Β© 2004 Society of Chemical Industry
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