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Screening of polymeric supports and enzymes for the development of an endo enzyme cleavable linker

✍ Scribed by Ana Chiva; David E. Williams; Alethea B. Tabor; Helen C. Hailes

Book ID
104097622
Publisher
Elsevier Science
Year
2010
Tongue
French
Weight
322 KB
Volume
51
Category
Article
ISSN
0040-4039

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✦ Synopsis

Several polymeric supports possessing an ester moiety were prepared and a range of enzymes was investigated to hydrolyse the ester linkage and release a signalling group into solution for applications in immunoassays. Pseudomonas lipases were found to most readily cleave the solution-phase analogue and this observation translated well to the corresponding polymeric supports, where the most effective were PEGA resins and the LPOS support PEG-6000.

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