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Screening of PC and PMMA-binding peptides for site-specific immobilization of proteins

✍ Scribed by Yoichi Kumada; Sho Murata; Yasuyuki Ishikawa; Kazuki Nakatsuka; Michimasa Kishimoto


Book ID
118473827
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
509 KB
Volume
160
Category
Article
ISSN
0168-1656

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Proteins carrying genetically attached polyhistidine tails have been purified using affinity precipitation with metal chelates. DNA fragments encoding four or five histidine residues have been genetically fused to the oligomeric enzymes lactate dehydrogenase (Bacillus stearother- mophilus), 0-glucor