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Screening of High-Affinity scFvs From a Ribosome Displayed Library Using BIAcore Biosensor

✍ Scribed by Qing Yuan; Zhongkang Wang; Siji Nian; Youping Yin; Gang Chen; Yuxian Xia


Book ID
107564944
Publisher
Springer-Verlag
Year
2008
Tongue
English
Weight
359 KB
Volume
152
Category
Article
ISSN
0273-2289

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M13 phages that display random disulfide constrained heptapeptides on their gpIII proteins were used to select for high affinity ligands to hepatitis B core antigen (HBcAg). Phages bearing the amino acid sequences C-WSFFSNI-C and C-WPFWGPW-C were isolated, and a binding assay in solution showed that