Screening of Garlic Water Extract for Binding Activity with Cholera Toxin B Pentamer by NMR Spectroscopy – An Old Remedy Giving a New Surprise
✍ Scribed by Matteo Politi; Josué Alvaro-Blanco; Patrick Groves; Alicia Prieto; J. Antonio Leal; F. Javier Cañada; Jesús Jiménez-Barbero
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 135 KB
- Volume
- 2006
- Category
- Article
- ISSN
- 1434-193X
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✦ Synopsis
Abstract
Binding between a component of the crude hot water extract obtained from Allium sativum crushed bulbs (ASw) and cholera toxin B pentamer (CTB) was detected by STD NMR experiments. Bioassay‐oriented fractionation allowed the partial identification of a high molecular weight polysaccharide mainly composed of galactose as the bioactive complex against CTB. This work represents the first example of screening of a medicinal plant by NMR against a specific disease, and corroborates traditional medical uses of the species. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)