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Screening of cyclic peptide phage libraries identifies ligands that bind streptavidin with high affinities

โœ Scribed by Cass, Robert; Milligan, Daniel L.; Giebel, Lutz B.; Young, Dennis; Arze, Rafael; Johnson, Charles


Book ID
115528080
Publisher
American Chemical Society
Year
1995
Tongue
English
Weight
818 KB
Volume
34
Category
Article
ISSN
0006-2960

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M13 phages that display random disulfide constrained heptapeptides on their gpIII proteins were used to select for high affinity ligands to hepatitis B core antigen (HBcAg). Phages bearing the amino acid sequences C-WSFFSNI-C and C-WPFWGPW-C were isolated, and a binding assay in solution showed that