The interaction between HIV-1 protease and inhibitors has been studied with optical biosensor technology. Optimized experimental procedures and mathematical analysis permitted determination of association and dissociation rate constants. A sensor surface with native enzyme was unstable and exhibited
✦ LIBER ✦
Screening of Compounds Interacting with HIV-1 Proteinase Using Optical Biosensor Technology
✍ Scribed by Per-Olof Markgren; Markku Hämäläinen; U.Helena Danielson
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 256 KB
- Volume
- 265
- Category
- Article
- ISSN
- 0003-2697
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The interaction between HIV-1 protease and reversible inhibitors was studied by surface plasmon resonance biosensor technology. The steady-state binding level and the time course of association and dissociation could be observed by measuring the binding of inhibitors injected in a continuous flow of