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Screening of a xylanase high-producing strain and its rapid separation and purification

✍ Scribed by Zhengchu Liu; Xiaoyang Dai; Juzuo Zhang; Junfei Xu; Shengwen Duan; Ke Zheng; Xiangyuan Feng; Lifeng Cheng; Jun Shi

Publisher
Springer-Verlag
Year
2011
Tongue
English
Weight
188 KB
Volume
61
Category
Article
ISSN
1590-4261

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Streptornyces lividans 66 was isolated from a clone obtained by shotgun cloning through functional complementation of a xylanase-and cellulase-negative mutant using the multicopy vector plJ702. This enzyme, designated xylanase C, has a relative molecular mass of 22 000 and acts on xylan similarly to