Screening of a synthetic peptide library against glycosomal phosphoglycerate kinase ofTrypanosoma brucei

✍ Scribed by Isabelle Samson; Arthur Aerschot; Bart Samyn; Jozef Beeumen; Piet Herdewijn

• Book ID: 104628418
• Publisher: Springer Netherlands
• Year: 1995
• Tongue: English
• Weight: 167 KB
• Volume: 2
• Category: Article
• ISSN: 1573-3149
• DOI: 10.1007/bf00119157

✦ Synopsis

A synthetic peptide library composed of polystyrene beads was prepared employing the split-synthesis approach, using the 20 natural L-amino acids except for cyste~ne. This library was screened for interaction with glycosomal phosphoglycerate kinase (gPGK) of T. brucei, labelled with fluorescein or with biotin. Affinity beads were individually subjected to Edman microsequence analysis. The corresponding peptide sequences were synthesized as free peptide acids and evaluated for enzyme activity inhibition. The pentapeptide NWMMF was able to selectively inhibit gPGK with an ICs0 of = 80 ~tM.