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Screening of a highly soluble and oxygen-independent blue fluorescent protein from metagenome

✍ Scribed by Chang-Sun Hwang; Eun-Sil Choi; Sang-Soo Han; Geun-Joong Kim


Book ID
116303084
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
934 KB
Volume
419
Category
Article
ISSN
0006-291X

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The origin of the low steady-state fluorescence quantum yield of some blue-emitting variants of the green fluorescent protein (GFP) is investigated in single-site mutants in which the tyrosine residue at position 66 has been replaced by phenylalanine or by histidine. Time-resolved fluorescence measu