Of eight white-rot fungi examined, seven fungi grew on nitrogen-limited poplar wood meal medium and degraded 14C-lignin in wood meal to 14CO2. Increased oxygen enhanced both the rate and extent of degradation. However, whereas Pleurotus ostreatus, Pycnoporus cinnabarinus 115 and Pycnoporus cinnabari
Screening for lignin degrading bacteria by means of14C-labelled lignins
β Scribed by K. Haider; J. Trojanowski; V. Sundman
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 339 KB
- Volume
- 119
- Category
- Article
- ISSN
- 0302-8933
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β¦ Synopsis
Several Nocardia and Pseudomonas spp., as well as some unidentified bacteria, isolated from lake water containing high loads of waste lignin, were tested for their capacity to release ~4CO2 from specifically 14C-labelled dehydropolymer of coniferyl alcohol (DHP) or corn stalk lignins. The bacteria were selected according to their ability to degrade phenolic compounds. However, only some of them could release significant amounts of 14CO2 from the labelled lignin. The tested Nocardia spp. were more active than the Pseudomonas spp. and the unidentified bacteria. The most active strains belonged to N. autotrophica. These strains released COzsignificantly from the methoxyl group and transformed the other cat'bons from the phenylpropane skeleton of lignin also into CO 2. Other less demethylating strains also released little CO 2 from the other carbons of the lignin molecule. From corn stalk materials which were specifically labelled in the lignin part, only small amounts of labelled CO 2 were released.
π SIMILAR VOLUMES
A Gram-positive bacterium which was isolated from a Finnish soil and identified as a Nocardia sp., was able to decompose lignin and to assimilate lignin degradation products as a carbon source. It could release 14COz from 14C-labelled methoxyl groups, side chains or ring carbons of coniferyl alcohol
The biodegradation of lignin by fungi was studied in shake flasks using 14C-labeled kraft lignin and in a deep-tank fermentor using unlabeled kraft lignin. Among the fungi screened, A. furnigatus-isolated in our laboratorieswas most potent in lignin biotransformation. Dialysistype fermentation, desi