Screening for improved cell performance: Selection of subclones with altered production kinetics or improved stability by cell sorting

Yeast-mediated stereo-selective reductio

Yeast-mediated stereo-selective reduction of estrone by continuous cell culture with dual stirred tanks for product yield improvement

✍ Cheanyeh Cheng; Hsiang-Rong Tsai 📂 Article 📅 2011 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 230 KB

## Abstract **BACKGROUND:** β‐Estradiol is an important hormone for the treatment of breast cancer and osteoporosis. β‐Estradiol can be produced via __Saccharomyces cerevisiae__ mediated reduction of estrone. However, substrate inhibition and low production yield have been observed in the batch cel