✍ Scribed by Alina Avramescu; Thierry Noguer; Maria Avramescu; Jean-Louis Marty
No coin nor oath required. For personal study only.
Biosensors for d-lactate and acetaldehyde were developed, based on screen-printed electrodes and NAD + -dependent dehydrogenases. Modification of screen-printed electrodes with the mediator Meldola Blue or with Meldola Blue-Reinecke salt resulted in sensitive, low cost and reliable NADH detectors. The biosensors were realised in two configurations, as disposable and reusable devices. Single-use sensors were obtained by simple deposition of enzyme and cofactor on the surface of mediator-modified electrodes. Chronoamperometry was used for the detection of substrates in small volumes of samples (25 l). Immobilisation of dehydrogenases by entrapment in poly(vinyl alcohol) bearing styrylpyridinium groups (PVA-SbQ) allowed sensors to be obtained with sufficient operational stability. Amperometry in stirred solutions was the detection technique with biosensors for multiple use. The 3σ detection limits for acetaldehyde were 1 M by amperometry and 6 M by chronoamperometry and for d-lactate-0.03 M and 0.05 M for reusable and disposable biosensors respectively.
The biosensors were applied in the analysis of some French and Romanian wines.
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