## Abstract Rat sciatic nerve Schwann cells either do not proliferate, or proliferate very slowly, in medium containing 10% fetal bovine serum (FBS). They were previously shown to respond only to a limited number of mitogens associated with cells of central and peripheral nervous systems, which app
Schwann cell responses to forskolin and cyclic AMP analogues: comparative study of mouse and rat Schwann cells
โ Scribed by Hitoshi Yamada; Atsushi Komiyama; Kinuko Suzuki
- Book ID
- 113127459
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 834 KB
- Volume
- 681
- Category
- Article
- ISSN
- 0006-8993
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๐ SIMILAR VOLUMES
## Abstract We present here a fast protocol that could be used to obtain highly purified cultures of maximal proliferating adult rat Schwann cells. These adult rat Schwann cells can be transfected in a nonbiological way using the physical transfection method of electroporation. Schwann cells are de
Na ฯฉ /K ฯฉ -ATPase-dependent Rb ฯฉ uptake of RN22 Schwann cells was stimulated by cholera toxin (0.25 ยตg/ml), forskolin (2 mM), or 8-bromo cAMP (1 mM). At 2 h Rb ฯฉ uptake was increased by 162 ฯฎ 6% (cholera toxin), 151 ฯฎ 14% (forskolin), and 207 ฯฎ 15% (8-bromo cAMP). Cholera toxin or 8-bromo cAMP treat