𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Scatter factor promotes motility of human glioma and neuromicrovascular endothelial cells

✍ Scribed by Katrin Lamszus; Nils Ole Schmidt; Liang Jin; John Laterra; David Zagzag; Dennis Way; Marlys Witte; Martin Weinand; Itzhak D. Goldberg; Manfred Westphal; Eliot M. Rosen

Publisher
John Wiley and Sons
Year
1998
Tongue
French
Weight
309 KB
Volume
75
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Malignant gliomas are characterized by rapid growth, infiltration of normal brain tissue, and high levels of tumorassociated angiogenesis. The genetic and local environmental tissue factors responsible for the malignant progression from low to high grade gliomas and the highly malignant behavior of glioblastomas are not well understood. In a study of 77 human brain tissue extracts, high grade (III-IV) tumors had significantly greater scatter factor (SF) content than did low grade tumors or non-neoplastic tissue. To investigate the potential significance of SF accumulation in gliomas, we measured the effects of SF on DNA synthesis and motility of cultured human glioma cell lines. SF stimulated DNA synthesis in 7/10 glioma cell lines and in 3/3 neuromicrovascular endothelial cell (NMVEC) lines, consistent with our previous report that SF stimulated cell proliferation of a few human glioma cell lines. SF markedly stimulated the chemotactic migration of 10/10 glioma cell lines as well as 3/3 NMVEC lines. In addition, SF stimulated the 2-dimensional migration of glioma cells on culture surfaces coated with specific extracellular matrix molecules (collagen IV, laminin, and fibronection). As expected based on these biologic responses to SF, 10/10 glioma lines and 4/4 NMVEC lines expressed mRNA for c-met, the SF receptor. To assess the possible in vivo significance of these migration assays, we compared the chemotactic response of a glioma cell line to human brain cyst fluids and tumor extracts that contained high or low SF concentrations. Fluids and extracts with high SF content tended to induce higher levels of chemotactic migration than did fluids and extracts with low SF content. Addition of anti-SF monoclonal antibody (MAb) inhibited migration induced by fluids and extracts with high SF content by about 30-50%. Int.

πŸ“œ SIMILAR VOLUMES

Effects of hepatocyte growth factor (HGF
Effects of hepatocyte growth factor (HGF) on human glioma cells in vitro: HGF acts as a motility factor in glioma cells
✍ Takuzou Moriyama; Hiroaki Kataoka; Kohji Seguchi; Hirohito Tsubouchi; Masashi Ko πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 French βš– 996 KB

Expression of c-Met, the receptor for hepatocyte growth factor (HGF). and the biological roles of HGF were examined in cultured human glioma cells. All of the 5 glioma cell lines examined expressed c-Met protein as well as the c-met gene. Expression of the c-met gene was also confirmed in a glioblas

Effect of scatter factor on motility of
Effect of scatter factor on motility of epithelial cells and fibroblasts
✍ Michael Stoker πŸ“‚ Article πŸ“… 1989 πŸ› John Wiley and Sons 🌐 English βš– 621 KB

The scatter factor is a protein released by fibroblasts that causes dispersal of epithelial cell colonies and disruption of intercellular junctions, as well as an alteration of morphology with ruffling and rapid extension and movement of pseudopodia. To find out if the scatter factor has a direct ef

Somatostatin inhibits the production of
Somatostatin inhibits the production of vascular endothelial growth factor in human glioma cells
✍ Rolf Mentlein; Olaf Eichler; Frauke Forstreuter; Janka Held-Feindt πŸ“‚ Article πŸ“… 2001 πŸ› John Wiley and Sons 🌐 French βš– 151 KB πŸ‘ 1 views

In various cell types, the neuro-and endocrine peptide somatostatin induces inhibitory and anti-secretory effects. Since somatostatin receptors, especially of the subtype sst2A, are constantly over-expressed in gliomas, we investigated the influence of somatostatin and the receptor subtype-selective

Isolation and culture of human neuromicr
Isolation and culture of human neuromicrovascular endothelial cells for the study of angiogenesis in vitro
✍ Katrin Lamszus; Nils Ole Schmidt; SΓΌleyman ErgΓΌn; Manfred Westphal πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 795 KB

Neovascularization in the adult central nervous system occurs as a response to several pathophysiological conditions such as ischemia, wound repair, or neoplasia. Endothelial cells from different blood vessel types, different organs, and different species are heterogeneous; therefore, the appropriat

CCL5/CCR5 axis promotes the motility of
CCL5/CCR5 axis promotes the motility of human oral cancer cells
✍ Jing-Yuan Chuang; Wei-Hung Yang; Hsien-Te Chen; Chun-Yin Huang; Tzu-Wei Tan; Yuh πŸ“‚ Article πŸ“… 2009 πŸ› John Wiley and Sons 🌐 English βš– 367 KB

## Abstract CCL5 (previously called RANTES) is in the CC‐chemokine family and plays a crucial role in the migration and metastasis of human cancer cells. On the other hand, the effect of CCL5 is mediated via CCR receptor. RT‐PCR and flow cytometry studies demonstrated CCR5 but not CCR1 and CCR3 mRN

Levels of vascular endothelial growth fa
Levels of vascular endothelial growth factor, hepatocyte growth factor/scatter factor and basic fibroblast growth factor in human gliomas and their relation to angiogenesis
✍ Nils Ole Schmidt; Manfred Westphal; Christian Hagel; SΓΌleyman ErgΓΌn; Dimitrios S πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 French βš– 288 KB πŸ‘ 2 views

Angiogenesis is a possible target in the treatment of human gliomas. To evaluate the role of 3 growth factors, vascular endothelial growth factor (VEGF), hepatocyte growth factor/scatter factor (HGF/SF) and basic fibroblast growth factor (bFGF), in the angiogenic cascade, we determined their levels