Scanning of polyacrylamide gel electrophoresis columns for detection of unstained protein zones and for their localization relative to enzyme activities

A Method for Activity Staining after Nat

A Method for Activity Staining after Native Polyacrylamide Gel Electrophoresis Using a Coupled Enzyme Assay and Fluorescence Detection: Application to the Analysis of Several Glycolytic Enzymes

✍ Jean Rivoal; Christopher R. Smith; Trevor F. Moraes; David H. Turpin; William C. 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 129 KB

We describe a method for the detection of isoforms of several glycolytic enzymes by activity staining after native PAGE. The staining is based on coupled enzyme assays carried out on the gel after electrophoresis and is linked to the disappearance of NADH, which is visualized by fluorescence. This m