## Beutenbergstrae Karyotypes of human metaphase chromosomes are used to detect genetic defects like deletions or translocations. For these investigations the chromosomes are treated by the trypsin-Giemsa protocol, resulting in a typical banding pattern. These patterns are investigated using conven
Scanning Near-field Optical Microscope Designed for Operation in Liquids
✍ Scribed by Mertesdorf, M.; Sch�nhoff, M.; Lohr, F.; Kirstein, S.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 348 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0142-2421
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✦ Synopsis
We report on the construction of a scanning near-Ðeld optical microscope (SNOM), which has been designed to permit imaging at the solid/liquid interface. The probe consists of a tapered Ðbre tip and the so called lateral shear force is used for the tip-sample distance regulation. The liquid environment causes damping of the tip vibration and lowering of the resonance frequency by approx. 20% which can not be explained by the model of a harmonic oscillator. We demonstrate the optical and topographical resolution of the instrument utilizing test grids for atomic force microscopy. First results of in-situ observation of adsorption of human serum albumin proteins at a thin Ðlm of polystyrene from aqueous bu †er solution are presented.
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We present aperture probes based on non-contact silicon atomic force microscopy (AFM) cantilevers for simultaneous AFM and near-infrared scanning near-Ðeld optical microscopy (SNOM). For use in high-resolution near-Ðeld optical microscopy, conventional AFM cantilevers are modiÐed by covering their t