Scanning-integrating cytophotometric analyses of brain neuronal RNA and acetylcholinesterase in acute soman toxicated rats
✍ Scribed by A. Anthony; J. A. Doebler; T. M. A. Bocan; C. Zerweck; T.-M. Shih
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 788 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0263-6484
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✦ Synopsis
Cytophotometric analyses of R N A and acetylcholinesterase responses of caudate and cerebrocortical neurons of soman toxicated rats were conducted to characterize impairments in regulatory aspects of neuronal metabolism occurring in the acute phase of cholinesterase impairment. There was a severe and dose-dependent suppression (20-60%) in neuronal acetylcholinesterase activity in both a.m. and p.m.treated rats; n o diurnal differences were apparent in control acetylcholinesterase levels or neuronal acety lcholinesterase responsiveness to soman toxication. R N A levels, however, were markedly higher in p.m. than in a.m. saline-treated controls, Soman depressed caudate neuron R N A contents in the afternoon, but not in the morning.
Cerebrocortical neuron R N A levels were suppressed in both a.m. and p.m.-toxicated rats, although this R N A depletion was more severe in the afternoon. These results indicate that soman can elicit marked alterations in neuronal transcriptional-translational capabilities and that there are diurnal variations in cellular metabolic responsiveness to soman toxication. Although functional relationships between somaninduced cholinesterase inhibition and R N A depletion remain to be elucidated, depressed R N A metabolism appears to be a maladaptive response preventing rapid regeneration of cholinesterase following poisoning.