Scanning Force Microscopy and Geometric Analysis of Two-Dimensional Collagen Network Formation

✍ Scribed by Mertig, M.; Thiele, U.; Bradt, J.; Leibiger, G.; Pompe, W.; Wendrock, H.

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 481 KB
• Volume: 25
• Category: Article
• ISSN: 0142-2421
• DOI: 10.1002/(sici)1096-9918(199706)25:7/8<514::aid-sia261>3.0.co;2-d

✦ Synopsis

Monomeric collagen Ðlms were prepared by spin-coating of acidic collagen solutions on di †erent atomically Ñat surfaces. The thin biomolecular coatings have been investigated by scanning force microscopy. Depending on both the wetting behaviour and the microtopology of the substrates used, di †erent Ðlm morphologies have been observed. Collagen monomers cover the surface of hydrophilic substrates homogeneously, whereas pore formation due to dewetting processes takes place at non-structured hydrophobic surfaces. The size of pores depends on the evaporation velocity of the solvent during spin-coating. Topological and metric properties of the resulting networks have been analysed and compared to soap foam network structures.

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