𝔖 Bobbio Scriptorium
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Scanning electron microscopy of piliatedNeisseria gonorrhoeae processed with hexamethyldisilazane

✍ Scribed by Dekker, Nusi P. ;Lammel, Claudia J. ;Brooks, Geo. F.

Publisher
Wiley (John Wiley & Sons)
Year
1991
Tongue
English
Weight
841 KB
Volume
19
Category
Article
ISSN
0741-0581

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✦ Synopsis

Piliated Neisseria gonorrhoeae are virulent and attach readily to some human mucosal cells. The study of interactions between piliated Neisseriu gonorrhoeae and surface structures of eukaryotic cells in tissue culture requires consistent high resolution imaging in scanning electron microscopy (SEM). The combination of the fixatives glutaraldehyde, osmium, tannic acid, and uranyl acetate improves preservation of pili and other delicate structures. Following the critical point drying (CPD) process, pili bundles remained intact, but charging produced image distortion in most of the specimens. The use of hexamethyldisilazane (HMDS) with air drying substantially reduced charging and image distortion. Less contrast and greater resolution of pili bundles and surface structures of bacteria or tissue culture cells were obtained at magnifications of 10,000 or higher. As an alternative to CPD, HMDS processing of cell culture monolayers was simple and was more efficient when a large number of samples was processed.

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