✦ LIBER ✦
Scanning cytophotometric analysis of myocardial nucleic acid and chromatin changes in soman toxicated rabbits
✍ Scribed by Lee J. Martin; Jeffrey A. Doebler; John W. Swisher; Dr. Adam Anthony; Tsung-Ming Shih
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 644 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0263-6484
No coin nor oath required. For personal study only.
✦ Synopsis
Myocardial nucleic acid responses were analysed in New Zealand White rabbits 20 min-1 h and 6 4 h following single subcutaneous injections of soman (20, 30, or 40 pg kg-'). Scanning-integrating microdensitometry was used to quantify Azure B -R N A and Feulgen-DNA (F-DNA) levels, and changes in the susceptibility of chromatin to Feulgen acid hydrolysis (F-DNA reactivity) of individual ventricular myocardial cells. With a dosage of 20 pg kg-I soman, no R N A alterations were evidenced at 1 h whereas at 6-8 h myocardial cells exhibited higher R N A levels and an increase in F-DNA reactivity of chromatin. With dosages of 30 and 40 pg kg-' soman there was an augmentation in R N A levels and i n the acid hydrolysability of nuclear chromatin at both 20 min-1 h and 6-8 h.
It is postulated that th,e observed cellular transformations represent a compensatory augmentation in myocardial metabolic functioning presumably in response to an increased functional demand on the ventricular myocardium. The absence of cytopathic or cytochemical evidence of impairment in nucleic acid metabolism is inconsistent with the premise that soman exerts direct cytotoxic effects on rabbit myocardium.