Multiparameter laser scanning cytometry has been applied to the automatic counting of probe spots and the simultaneous measurement of cellular DNA for fluorescence in situ hybridization (FISH) prepared specimens counterstained with propidium iodide. Relatively low resolution imaging, highly variable
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Scanning cytometry with a LEAP: Laser-enabled analysis and processing of live cells in situ
โ Scribed by Peter Szaniszlo; William A. Rose; Nan Wang; Lisa M. Reece; Tamara V. Tsulaia; Elie G. Hanania; Cornelis J. Elferink; James F. Leary
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 373 KB
- Volume
- 69A
- Category
- Article
- ISSN
- 0196-4763
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## Background: A recently developed laser scanning cytometry technique was applied to cytometric studies to detect rapidly stable chromosomal aberrations induced by a carcinogen in a chinese hamster fibroblast cell line, cho-wblt. ## Methods: Individual chromosomes were collected from metaphase c