Scaling-up procedure from the range of milligrams to grams for the purification of amino acid derivatives in displacement chromatography

✍ Scribed by F. Cardinali; A. Ziggiotti; G.C. Viscomi

• Book ID: 104144957
• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 708 KB
• Volume: 499
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)96961-9

✦ Synopsis

A scaling up method in displacement chromatography was applied to the purification of the amino acid derivative N"-9-fluorenoxycarbonyl-S-tritylcysteine [Fmoc-Cys(Trt)-OH].

Once the adsorption isotherm of the compound and some related impurities had been determined, the displacement chromatographic runs were carried out in the normal phase. The influence of parameters such as particle diameter of the stationary phase, carrier composition, nature of the displacer, concentration of the loaded sample and flow-rate is reported. The eluates were analysed directly at the outlet of the preparative columns with dedicated on-line detection equipment. The results demonstrate that, once the proper conditions have been established, it is possible to purify in one run 100 mg and 38 g of feed mixture by proportionally increasing the amount of the stationary phase, using a larger column and maintaining the linear velocity and displacer concentration constant.