✦ LIBER ✦
Scaled particle theory applied to gels of high molecular weight DNA in the ultracentrifuge
✍ Scribed by Alfred J. Richard; Richard B. Westkaemper
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1986
- Tongue
- English
- Weight
- 606 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0006-3525
No coin nor oath required. For personal study only.
✦ Synopsis
Equilibrium ultracentrifugation of high molecular weight DNA a t moderate centrifuge speed leads to the formation of gel a t the bottom of the centrifuge cell. The swelling pressures of the gels are strongly affected by the ionic strength of the medium and by polyvalent counterions in the medium. Scaled particle theory applied t o the DNA gels gives values to the effective particle length and particle radius of the DNA. Different models for DNA conformations in the gels were required for the monovalent, the divalent, and the tetravalent cations used in this study.