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Scale-up of “myxalin” purification by a pseudoaffinity method using a radial flow column

✍ Scribed by A. Akoum; M. A. Vijayalakshmi; M. Sigot

Book ID
112731254
Publisher
Springer
Year
1989
Tongue
English
Weight
234 KB
Volume
28
Category
Article
ISSN
0009-5893

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The large-scale purification of plasmid DNA was achieved using fast protein liquid chromatography on a Hi-Load Q Sepharose column. This method allows for the purification of plasmids starting from crude plasmid DNA, prepared by a simple alkaline lysis procedure, to pure DNA in less than 5 h. In cont