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Sandwich immunoassay for the detection of lipoteichoic acid

✍ Scribed by Monica B. Tadler; George Tice; Susan M. Wos

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
452 KB
Volume
3
Category
Article
ISSN
0887-8013

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✦ Synopsis

A sandwich immunoassay has been developed for the detection of lipoteichoic acid (LTA), a major cell wall constituent of gram-positive bacteria, from whole blood and ISOLATOR supernate. Monoclonal antibodies were produced to purified LTA from Streptococcus mutans, BHT and were further characterized for crossreactivity with gram-positive and negative bacteria and for reactivity to substituted and unsubstituted LTA. Eight monoclonal antibodies were identified that reacted exclusively with gram-positive bacteria. Those antibodies able to capture 3H-LTA were chosen to develop a sandwich immunoassay. The assay has a sensitivity of 0.2 ng LTAlmL in PBS, 0.5 nglmL in whole blood and 2.0 ngl mL in whole blood that has been processed through the ISOLATOR. Further development of this assay may lead to the rapid detection of LTA from body fluids.

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