𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Sample stacking for the analysis of catechins by microemulsion EKC

✍ Scribed by Hsi-Ya Huang; I.-Yun Huang; Hsin-hui Liang; Szetsen Lee

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
478 KB
Volume
28
Category
Article
ISSN
0173-0835

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

In this study, an on‐line concentration method, ASEI (anion‐selective exhaustive injection)‐sweeping technology which was coupled with microemulsion EKC (MEEKC), was used to analyze and detect six catechins ((−)‐epicatechin, (+)‐catechin, (−)‐epigallocatechin gallate, (−)‐epicatechin gallate, (−)‐epigallocatechin, and (−)‐gallocatechin). In addition to the effects of the buffer pH and electrolyte concentration on stacking, the compositions of microemulsion (types of oil phase, and types and levels of cosurfactant) also dominated the stacking effect of catechins. In MEEKC, the effect of the type of oil in microemulsion on separation mechanism is often unclear. This study had demonstrated that the oil type in microemulsion indeed altered the affinity of oil droplets with analytes. Finally, this proposed ASEI‐sweeping MEEKC method was able to detect trace level of catechins in food products that was not previously possible by a normal MEEKC method.

📜 SIMILAR VOLUMES

Sample stacking for the analysis of eigh
Sample stacking for the analysis of eight penicillin antibiotics by micellar electrokinetic capillary chromatography
✍ Patricia Puig; Francesc Borrull; Marta Calull; Carme Aguilar 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 237 KB 👁 2 views

## Abstract We studied the use of micellar electrokinetic capillary chromatography for separating eight penicillins. The method consists of (i) an electrophoretic separation based on micellar electrokinetic capillary chromatography, which uses sodium dodecyl sulfate (SDS) as surfactant; (ii) a samp

Microemulsion electrokinetic chromatogra
Microemulsion electrokinetic chromatography for the analysis of green tea catechins: Effect of the cosurfactant on the separation selectivity
✍ Romeo Pomponio; Roberto Gotti; Barbara Luppi; Vanni Cavrini 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 107 KB 👁 1 views

## Abstract Microemulsion electrokinetic chromatography (MEEKC) was applied to the separation of six catechins and caffeine, the major constituents of the green tea. The developed methods involved the use of sodium dodecyl sulfate (SDS) as surfactant, __n__‐heptane as organic solvent and an alcohol