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Sample preparation of human tumors prior to two-dimensional electrophoresis of proteins

✍ Scribed by Dr. Bo Franzén; Takashi Hirano; Ken Okuzawa; Kazuto Uryu; Ayodele A. Alaiya; Stig Linder; Gert Auer

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
339 KB
Volume
16
Category
Article
ISSN
0173-0835

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✍ James R. Jefferies; Peter M. Brophy; John Barrett 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 830 KB

This paper investigates the preparation of Fasciola hepatica samples for two-dimensional electrophoresis (2-DE). Whole samples were prepared by both hot sodium dodecyl sulfate (SDS) solubilisation and precipitation using trichloroacetic acid (TCA) to remove nonprotein contaminants and to inactivate