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Sample-induced internal pH gradients in microcolumn liquid chromatography of proteins

✍ Scribed by M. Janeček; J. Šalamoun; K. Šlais

Book ID
112720571
Publisher
Springer
Year
1991
Tongue
English
Weight
325 KB
Volume
32
Category
Article
ISSN
0009-5893

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📜 SIMILAR VOLUMES

Microcolumn liquid chromatography with s
Microcolumn liquid chromatography with sample induced internal pH gradient
✍ Karel Šlais 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 619 KB

An internal pH gradient of a wide range of pH can be developed using the pumped mobile phase containing only a few [3][4][5][6] simple buffering salts which differ in pka values by about 1 pk, unit or less. The use of several types of surface ionizable groups allows the internal pH gradient to be ef