Salt- and solvent-dependent conformational transitions of ribo-CGCGCG duplex

Circular dichroism spectroscopy analysis

Circular dichroism spectroscopy analysis of conformational transitions of a 54 base pair DNA duplex composed of alternating CGCGCG and TATATA blocks

✍ Jaroslav Kypr; Jiří Štěpán; Jana Chládková; Michaela Vorlíčková 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 283 KB 👁 2 views

CD spectroscopy was used to analyze conformational properties of a selfcomplementary 54-mer DNA composed of alternating (CG) 3 and (TA) 3 hexamers. NaCl induced the Z-form in poly(GC), but the 54-mer remained the B-form under the same conditions. The B-Z transition was induced only after the additio