Safety assessment of genetically engineered potatoes with designed soybean glycinin: compositional analyses of the potato tubers and digestibility of the newly expressed protein in transgenic potatoes
✍ Scribed by Hashimoto, Wataru; Momma, Keiko; Katsube, Tomoyuki; Ohkawa, Yasunobu; Ishige, Teruo; Kito, Makoto; Utsumi, Shigeru; Murata, Kousaku
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 151 KB
- Volume
- 79
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
The compositions of transgenic potatoes with intact and modi®ed genes for soybean glycinin were compared with those of control potatoes; non-transgenic or transgenic ones with a vector. The expression levels of glycinin proteins in the transgenic potato tubers were approximately 12±31 mg g À1total soluble protein. With reference to six major components, moisture, protein, lipid, ®ber, ash and carbohydrate, there were no signi®cant differences between transgenic and control potatoes. The levels of glycoalkaloids in transgenic potato tubers, as well as in transgenic controls, increased in comparison with those in non-transgenic ones, though the level was within the permitted limit. The modi®ed glycinin expressed in the transgenic potato tuber was digested under the simulated gastric conditions. From these results, the transgenic potatoes with intact and modi®ed glycinin genes are considered to be as safety utilization for food as non-transgenic potatoes.