Safer derivatives of bacteriophage λgt.λC for use in cloning of recombinant DNA molecules
✍ Scribed by ENQUIST, L.; TIEMEIER, D.; LEDER, P.; WEISBERG, R.; STERNBERG, N.
- Book ID
- 109698305
- Publisher
- Nature Publishing Group
- Year
- 1976
- Tongue
- English
- Weight
- 401 KB
- Volume
- 259
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/259596a0
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A method is described for the purification of DNA from recombinant clones in lambda vectors. Plate lysates are used as the starting material, with the phage suspension being either loaded directly onto mini-cesium chloride two-step gradients or first subjected to precipitation with polyethylene glyc
We previously demonstrated that the system integrated into the host chromosome can overcome the instability encountered in continuous operations of unstable plasmid-based expression vectors. High stability of a cloned gene in a lysogenic state and a high copy number in a lytic state provide cloned-g