Saccharification of native and degraded cotton cellulose and commercial microcrystalline cellulose byTrichoderma viridecellobiohydrolase I

✍ Scribed by M. Finetti; M. Daz; G. Ellenrieder; M. Marx-Figini

• Publisher: Springer
• Year: 1993
• Tongue: English
• Weight: 350 KB
• Volume: 9
• Category: Article
• ISSN: 1573-0972
• DOI: 10.1007/bf00327849

✦ Synopsis

The degree of polymerization of samples of acid degraded cotton cellulose has no appreciable influence on the saccharification by cellobiohydrolase I from Trichoderma viride. The increase in the number of cellulose molecule ends, achieved by a 30-fold decrease in molecular weight, does not produce the effect which could be expected for a pure end-wise mode of action of this exoglucanase. Microcrystalline celluloses saccharified by the same enzyme yield considerably more reducing sugars than cotton cellulose, either with a similar degree of polymerization or one of about 7000. It appears, therefore, that the difference in the susceptibility of the commercial substrates is not a consequence of their low degree of polymerization.