Sa.74. Development of a Biomarker Assay Using ELISPOT for Detecting KLH Specific IFN-Y Secreting T-Cells

Detection and quantification of CD8+ T c

Detection and quantification of CD8+ T cells specific for HLA-A*0201-binding melanoma and viral peptides by the IFN-γ-elispot assay

✍ Marieke Griffioen; Martina Borghi; Peter I. Schrier; Susanne Osanto 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 French ⚖ 122 KB 👁 1 views

Blood lymphocytes from HLA-A\*0201-subtyped melanoma patients and healthy controls were screened for the presence of T cells specific for HLA-A\*0201-binding melanoma and viral peptide antigens by the enzyme-linked immunoSPOT (ELISPOT) assay. CD8(+) cells were tested for peptide-specific IFN-gamma r