Within the context of the hygiene hypothesis we aimed to study the potential association between farming related risk factors and Toxoplasma gondii (T gondii) seropositivity. Within in a cross-sectional study on allergic diseases in young adults living in a rural environment we analyzed serum samples of 106 cases with specific IgEs against a panel of common aeroallergens and 215 controls for T gondii seropositivity. Living on a farm, regular visits to animal stables in early childhood, as well as consumption of unpasteurized milk at age 6 were significantly associated with T gondii seropositivity in the crude analyses. In the multivariate model, regular contact to animal stables before age 3 was the strongest risk factor for T gondii infection (odds ratio (95% confidence interval): 2.1 (1.0-4.1)). T gondii seropositivity and farming related factors seemed to decrease the risk of atopy in an additive matter; i.e., subjects with T gondii seropositivity, regular contact to animal stables and consumption of unpasteurized milk during childhood were least likely to be atopic (0.4 (0.1-1.0); p<0.05). Exposure to farming environments in childhood and T gondii seropositivity might be closely related in rural subjects.