Combined use of micropipette aspiration
Combined use of micropipette aspiration and perifusion for studying red blood cell volume regulation
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K. Gunnar EngstrΓΆm; Herbert J. Meiselman
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Article
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1997
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John Wiley and Sons
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English
β 162 KB
We combined micropipette aspiration with a new technique of perifusion to study volume behavior of single red blood cells (RBCs) during anisotonic conditions. Other techniques have suggested that RBC volume changes are complete within 1 s, but our pipette data indicate a slower volume response of 5-