S-35-glutathione preparation from yeast and tracer studies in cancerous and non-cancerous rats

✍ Scribed by Gladys E. Woodward

• Book ID: 103080787
• Publisher: Elsevier Science
• Year: 1951
• Tongue: English
• Weight: 393 KB
• Volume: 251
• Category: Article
• ISSN: 0016-0032
• DOI: 10.1016/0016-0032(51)90600-x

✦ Synopsis

Glutathione (GSH) occurs in relatively high amounts in almost all living tissue yet its exact function has not been established. The use of S35-1abeled GSH in tracer studies has not previously been reported. In the present studies, S35-1abeled GSH has been prepared and administered to normal, protein-depleted and tumor-bearing rats, and the S a5 uptake in various tissues has been determined.

PREPARATION OF S~-LABELED GSH

The synthesis of S3~-labeled GSH in quantity sufficient for tracer studies had not been described, although Franklin (1) showed that radioactive GSH was present in yeast grown in a medium containing S 85 in the form of sulfate. In attempting to grow yeast on a scale sufficiently large to allow isolation of GSH, we found that certain cultures of yeast contained very little GSH but that the GSH concentration in yeast was regularly high if a low concentration of glucose and a high rate of aeration were maintained in the medium during growth of the yeast. Description of our most satisfactory procedure for production of radioactive GSH through biosynthesis by yeast is therefore given somewhat in detail.

The basic medium contained the following materials per liter of culture :