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Ryanodine-induced intracellular calcium mobilisation in cultured astrocytes

✍ Scribed by David Langley; Brian Pearce

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 685 KB
Volume: 12
Category: Article
ISSN: 0894-1491
DOI: 10.1002/glia.440120206

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✦ Synopsis

Abstract

Changes in intracellular Ca^2+^ concentration were monitored in cultured cortical astrocytes challenged with ryanodine and ATP. Ryanodine elicited a modest, in comparison to ATP, increase in cytosolic Ca^2+^ concentration in approximately 60% of the cell fields examined. This effect was evident and, in fact, was augmented when incubations were performed in Ca^2+^ concentration were dose dependent, desensitised rapidly, and could not be mimicked by caffeine. Exposure to ryanodine was without effect on eicosanoid release from these cells nor did it influence Ca^2+^ mobilisation and eicosanoid release in response to ATP. In contrast, caffeine attenuated part of the ATP‐evoked increase in intracellular Ca^2+^ concentration in the majority of cells tested and abolished its effect on eicosanoid release.

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