Rule of Intersection Point between Retention of Homologues and Column Temperature in Reversed-Phase Liquid Chromatography
β Scribed by F.A. Wang; J.C. Song; Y.C. Guo; Y.H. Wang; Y. Zhao
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 173 KB
- Volume
- 52
- Category
- Article
- ISSN
- 0026-265X
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β¦ Synopsis
The rule of intersection point between retention of homologues and column temperature in reversed-phase liquid chromatography is reported, based on the thermodynamic theory, with the basic equation of the retention in reversed-phase liquid chromatography, proposed by Vigh and others. We have derived the rule of intersection point between the logarithm of capacity factor and column temperature for homologues in reversed-phase liquid chromatography, and used the experimental data to verify it. The result is satisfactory. (C) 1995 Academic Press, Inc
π SIMILAR VOLUMES
The solute retention mechanism of reversed-phase liquid chromatography is examined by using newly synthesized hydrophilic porous copolymer beads as a stationary phase. It is shown that when water is used as an eluent, the retention time for solutes such as alcohols, ketones and polyethylene glycols