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RT-PCR for the pseudogene-free amplification of the glyceraldehyde-3-phosphate dehydrogenase gene (gapd)

โœ Scribed by Lucy V Harper; Anthony C Hilton; Alan F Jones


Book ID
117593205
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
101 KB
Volume
17
Category
Article
ISSN
0890-8508

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The gene coding for glyceraldehyde-3-phosphate dehydrogenase (gpd) has been isolated from a genomic library of the filamentous fungus Curvularia lunata. The coding region of this gene consists of 1014 nucleotides and is interrupted by four introns. The gpd gene product shows a high degree of sequenc