Rotavirus diarrhea in children and adults in a southern city of Brazil in 2003: Distribution of G/P types and finding of a rare G12 strain

✍ Scribed by Eduardo Pietruchinski; Fabrício Benati; Flávio Lauretti; Jonas Kisielius; Marli Ueda; Eduardo M. Volotão; Caroline C. Soares; Yasutaka Hoshino; Rosa Elisa C. Linhares; Carlos Nozawa; Norma Santos

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 179 KB
• Volume: 78
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.20686

✦ Synopsis

Abstract

Between May and August in 2003, a total of 251 fecal samples were collected from children and adults with diarrhea (5 inpatients and 246 outpatients) at a private hospital in the city of Ponta Grossa, the state of Paraná, Brazil. Group A rotavirus was detected in 71 of 251 (28.3%) specimens: 55 (77.5%) from children under 5 years of age and 16 (22.5%) from individuals aged 6–72 years. All 71 strains exhibited a “long” RNA pattern when analyzed by PAGE. Sixty‐one positive samples that yielded enough RNA were submitted to PCR genotyping. The most frequent G/P genotype combination detected was G1P[8] (86.9%; 53/61) followed by G9P[8] (3.3%; 2/61) and G12P[9] (1.6%; 1/61). Rotaviruses with G2, G3, G4, P[4], or P[6] specificity were not detected. For three strains (4.9%) bearing G1 genotype, the VP4 specificity could no be determined, and two specimens (3.3%) remained G/P non‐typeable. One rotavirus strain (HC91) bearing G12P[9] genotype with a “long” electropherotype was isolated from an 11‐month‐old boy with diarrhea for the first time in Brazil. The cell‐culture grown HC91 strain was shown to belong to serotype G12 by neutralization. J. Med. Virol. 78:1241–1249, 2006. © 2006 Wiley‐Liss, Inc.