The internuclear distance between two homonuclear dipolar-coupled dilute spins-l/2, S\* and Sx, can be measured by mtatianal resonance experiments in solid state NMR. These involve rotating the sample at the magic angle while fulfilling the condition for rotational resonance, f&-Q,= ncu,, where Q, a
Rotational resonance in solid state NMR
โ Scribed by D.P. Raleigh; M.H. Levitt; R.G. Griffin
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 534 KB
- Volume
- 146
- Category
- Article
- ISSN
- 0009-2614
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A solid-state nuclear magnetic resonance (NMR) technique is described for obtaining constraints on the backbone conformation of a protein or peptide that is prepared with uniform 15 N, 13 C labeling of consecutive pairs of amino acids or of longer segments. The technique, called double single-quantu
Simple modifications of the rotational resonance experiment substantially reduce the total experimental time needed to measure weak homonuclear dipolar couplings, a critical factor for achieving routine internuclear distance measurements in large biomolecular systems. These modifications also addres